Detection of human ATM protein using anti-ATM 2C1 monoclonal antibody (3310) by western blot or immunoprecipitation.

Human Kidney (formalin-fixed, paraffin-embedded) stained with ATM antibody at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody [2C1] (3310) diluted at 1:1000.

HeLa whole cell extract and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody [2C1] (3310) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.